COMPASSION. EXPERIENCE. RESPECT.

We Clean Tough Situations: Charlotte Suicide, Crime Scene, Hoarding, and Biohazard Cleanup

Bio-One of Charlotte helps families and communities after life’s most challenging moments. We clean what others can’t—biohazards, trauma, crime, and suicide scenes, hoarding, and more.

Our trained team shows up fast, works with care, and treats your space with respect.

  • Available 24/7.
  • Discreet arrival—unmarked vehicles upon request.
  • We work with insurance.
  • Locally owned and trusted.

Real help for real life: let us handle the cleanup, so you can focus on the path ahead.

Emergency response hotline

Get in touch with our 24/7 biohazard & crime scene cleaning team today

Call me directly:John and Jen Symons
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Jeff H.

John and Jen did an outstanding job with our attic area. They removed all debris, applied disinfectant And replaced the insulation for a very reasonable price. They are on time, profess...

Christie T.

Thank you so much for the recent job at my home! I am grateful to have found your company. I appreciate the consideration for my feelings and care toward the special items in my home. E...

Randy C.

Absolutely one of the finest companies we have ever hired to do work. They were an A+++++++++++ . They took our hoarded house we inherited and cleaned it out to make it look presentable...

Holly Martha Holland Belue

Very fast turnaround. Especially helpful with insurance claim. Thank you so much..we highly recommend this company.

Jennifer P.

AWESOME HAZMAT SERVICE. EVERYTHING WAS FAST WITH EXCELLENT COMMUNICATION!

Trusted By
8 Hour Refresher Certificate
First Aid USA Certificate
Mold Remediation Certificate
Decontect Certificate

Where Crisis Meets Compassion

Bio-One responds when families need more than just cleanup—they need someone who truly cares.
Five Star Experience
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Help first. Business second.

We provide compassionate, discreet biohazard cleanup, restoring safety and peace of mind in times of crisis.
Suicide Cleanup Icon

Suicide Cleanup

We clean with care, helping families begin to heal.

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Crime Scene Cleanup Icon

Crime Scene Cleanup

We remove hazards so you can move forward safely.

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Blood Spill Cleanup Icon

Blood Spill Cleanup

We clean thoroughly to restore safety and peace of mind.

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Hoarding Cleanup Icon

Hoarding Cleanup

We are here to earn your trust and ease you into the hoarding cleanup process.

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Decomposition / Undiscovered Death Icon

Decomposition / Undiscovered Death

We handle the toughest scenes with dignity and compassion.

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Medical Waste Disposal Icon

Medical Waste Disposal

We offer medical waste disposal and train others on its safe handling.

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Emergency Vehicle Decontamination Icon

Emergency Vehicle Decontamination

We help protect first responders with deep, professional cleaning.

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Odor Removal Icon

Odor Removal

We will find the source and remediate it.

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View All Services

We respond 24/7.
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Bio-One of Charlotte

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    WE'RE READY 24/7

    Work with us

    Three simple steps. One compassionate team.
    1

    Call Anytime—Day or Night

    Reach out 24/7. A kind, trained expert will listen and guide you through the next steps.
    2

    On-Site, With Care

    We’ll arrive quickly, walk the space with you, and create a plan that fits your needs—with total discretion and respect.
    3

    You're in Good Hands

    We’ll handle the cleanup with care and follow through until you’re fully satisfied. We can even work with your insurance to ease the process.

    Ready For Anything 24/7/36

    Three simple steps. One compassionate team.
    Always here. Always ready. No matter the hour, you’ll reach someone who listens, cares, and knows how to help.

    Call us anytime—24/7/365—for fast, compassionate service from a local Bio-One expert.
    Schedule a Service
    Why choose Bio-One of Charlotte?

    Trusted When It Matters Most

    Because you deserve more than just a cleanup—you deserve care.

    Families, property owners, and first responders choose Bio-One for fast, discreet, and compassionate cleanup.

    People call us during some of their most challenging moments. We show up fast, listen first, and handle every scene skillfully and compassionately.

    • Fast, local 24/7 response
    • Trained experts who treat you with respect
    • Discreet, thorough cleanup from start to finish
    • We handle the insurance, so you don’t have to

    We’re here to help you feel safe again—and we’ll walk with you every step of the way.

    Call Us 27/4
    Your Local Cleanup Partner

    Rooted in the community. Driven by compassion.

    The person behind your local Bio-One isn’t just a business owner—they’re a neighbor who cares deeply about helping others through life’s most challenging moments. Trained, certified, and ready to respond 24/7, they lead a team committed to doing the right thing with discretion, empathy, and professionalism.

    You can trust that when you call, you get real help from someone who truly understands.

    John and Jen Symons

    John and Jen Symons are the owners of Bio-One of Charlotte. Both John and Jen grew up in Michigan and moved to Charlotte, NC in 2007. John has worked in the restoration field for over ten years, and loves helping his customers. He uses his experience and expertise to help restore their client's homes to a safe and clean condition. Jen graduated from Central Michigan University and is passionate about helping those in need. John and Jen have two daughters and are very active in their community of Rock Hill, SC.
    Learn more about me and my mission
    Call Us 24/7

    Real People. Real Compassion.

    Our local team is trained, experienced, and here to help—no judgment, just support.

    We understand that calling us means something serious has happened. That’s why our local Bio-One team brings more than training—we bring compassion, clear communication, and respect every step of the way.

    • Highly trained and experienced technicians
    • Honest, in-person estimates
    • Discreet and professional service
    • Locally owned and operated

    You can count on us to handle it all—with care.

    Meet my team

    What our clients say

    decontamination and biohazard cleaning services reviews
    J B, Charlotte, 05/2022
    Bio-One reps were very considerate and helpful with me as we worked through a very difficult family situation. They went above and beyond to treat us like valued customers and job was very well done. Service: Deep clean
    decontamination and biohazard cleaning services reviews
    GHC Building Maintenance, Charlotte, 05/2022
    decontamination and biohazard cleaning services reviews
    Martha, Charlotte, 04/2022
    They removed a house full of junk and old furniture, including an upright piano, and disposed in dumpster. They found a lot, a lot of loose change, which they handed over to me. They cleaned out the kitchen cabinets, wiped down all the interiors and exteriors of cabinets, wiped down all appliances, counters, light fixtures, floors, walls, bathrooms. It was all done efficiently and with care in about a day and a half. They kept me posted on when they would be there and when they were leaving. Really professional and very nice crew
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    COMMUNITY

    Rooted in the Community, Ready to Help

    We live here, we care, and we’re proud to serve our neighbors.

    We love the people, places, and spirit of the communities we serve. That’s why we show up 24/7—to help our neighbors through tough moments with care, compassion, and respect.

    From local families to first responders, we’re honored to be a trusted part of the community.
    Serving Charlotte and Surrounding Areas
    Find your nearest Bio-One office

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    Frequently asked questions

    What types of situations does Bio-One handle?
    We clean biohazards, trauma scenes, suicides, unattended deaths, hoarding situations, and more—discreetly and with care.
    Are you really available 24/7?
    Yes. No matter the time or day, someone from our local team is always ready to answer your call.
    Will my information be kept private?
    Absolutely. Upon request, we arrive in unmarked vehicles and treat every situation with total discretion and respect.
    How quickly can you arrive?
    We respond quickly—usually the same day. We’re locally owned, so we can get to you quickly.
    Do you work with insurance?
    Yes. We’ll work directly with your insurance provider to help make the process easier for you.
    What happens when I call Bio-One?
    A kind, trained expert will listen, explain the next steps, and guide you through the process—no pressure, no judgment.
    Is your team trained for hazardous cleanup?
    Yes. Our technicians are fully trained, certified, and experienced in handling dangerous and sensitive situations.
    Do I have to be home during the cleanup?
    Not if you don’t want to be. We’ll work with your schedule and comfort level every step of the way.
    How long does cleanup usually take?
    It depends on the situation, but most cleanups take a day or two. We’ll give you a clear timeline upfront.
    Why should I trust Bio-One?
    Because we genuinely care. We’re part of your community and show up with compassion, professionalism, and respect—every time.
    24/7 EMERGENCY HOTLINE
    Schedule a Service

    Get in touch with our 24/7 biohazard & crime scene cleaning team today

    Bio-One of Charlotte

    Charlotte Decontamination and Biohazard Cleaning Services

    13000 South Tryon St. Suite 232-F , Charlotte, NC 28278
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    ©️2022-2026