COMPASSION. EXPERIENCE. RESPECT.

Professional Crime Scene Cleanup & Decontamination Service in Charlotte

With expertise in emergency services cleanup and decontamination services in Charlotte for more than 20 years, Bio-One’s team of cleanup professionals handles every situation with care, restoring affected areas safely and thoroughly. That’s because we’re invested in your well-being, backed by experience, and equipped with personalized cleaning plans for any situation.

With our Charlotte trauma cleanup solutions, we stay on-site long after first responders and authorities have gone. We understand the business we’re in and how emotional it can be when losing a loved one or parting with your possessions during a crisis.

From odor removal to biohazard cleanup services throughout Charlotte, our mission is to make spaces safe again for you and the people you value … so you can focus on what matters most: healing.

Emergency response hotline

Get in touch with our 24/7 biohazard & crime scene cleaning team today

Call me directly:John and Jen Symons
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MAXIE GASTON

Thank you so much for providing a proactive COVID-19 cleaning to my home.

Don Kaleel

Prompt, professional, on time, did a good job, and reasonable.

Karen Arnold

Great people running this company - caring, compassionate, and very good service

Lindsey Mullis

This company is wonderful! I cannot say enough good things about them. They were thorough, efficient, and incredibly nice.

Louis Smith

Quick service. Quick Response! thank you!

Trusted By
8 Hour Refresher Certificate
First Aid USA Certificate
Mold Remediation Certificate
Decontect Certificate

Where Crisis Meets Compassion

Bio-One responds when families need more than just cleanup—they need someone who truly cares.
Five Star Experience
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Help first. Business second.

We provide compassionate, discreet biohazard cleanup, restoring safety and peace of mind in times of crisis.
Suicide Cleanup Icon

Suicide Cleanup

We clean with care, helping families begin to heal.

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Crime Scene Cleanup Icon

Crime Scene Cleanup

We remove hazards so you can move forward safely.

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Blood Spill Cleanup Icon

Blood Spill Cleanup

We clean thoroughly to restore safety and peace of mind.

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Hoarding Cleanup Icon

Hoarding Cleanup

We are here to earn your trust and ease you into the hoarding cleanup process.

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Decomposition / Undiscovered Death Icon

Decomposition / Undiscovered Death

We handle the toughest scenes with dignity and compassion.

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Medical Waste Disposal Icon

Medical Waste Disposal

We offer medical waste disposal and train others on its safe handling.

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Emergency Vehicle Decontamination Icon

Emergency Vehicle Decontamination

We help protect first responders with deep, professional cleaning.

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Odor Removal Icon

Odor Removal

We will find the source and remediate it.

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We respond 24/7.
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Bio-One of Charlotte

    COMPASSION. EXPERIENCE. RESPECT.

    Work with us

    Three simple steps. One compassionate team.
    1

    Call Anytime—Day or Night

    Reach out 24/7. A kind, trained expert will listen and guide you through the next steps.
    2

    On-Site, With Care

    We’ll arrive quickly, walk the space with you, and create a plan that fits your needs—with total discretion and respect.
    3

    You're in Good Hands

    We’ll handle the cleanup with care and follow through until you’re fully satisfied. We can even work with your insurance to ease the process.

    Discrete & Fast Emergency Services & Charlotte Trauma Clean Up When You Need It

    Three simple steps. One compassionate team.

    Our specialized cleaning and biohazardous decontamination services cater to your needs quickly and efficiently in the following ways: 

    Here’s a step-by-step guide for how we take care of your personal or professional space:

    1. You’ll call as soon as you need assistance: With our dedicated and confidential teams working 24 hours a day, 7 days a week, we always answer your call when your life gets overwhelming. 
    2. We’ll provide you with comprehensive remediation: Our friendly and responsive team will schedule an appointment with you and then arrive at your residence or premises within the hour. From there, we’ll clean up or decontaminate the area carefully and thoroughly from start to finish. 
    3. We’ll work closely with your insurance provider: No one understands better than we do how stressful and time-consuming working with insurance companies can be. That’s why we open an insurance claim and work directly with the adjusters so you can focus on what matters most to you.

    If you need specialized Charlotte biohazard cleanup services and more caused by highly traumatic, life-changing situations, then we’re here to lend a helping hand.

    Schedule a Service
    Why choose Bio-One of Charlotte?

    Why Bio-One of Charlotte is the Best Team for the Job

    When it comes to a deep-rooted passion for helping those in need, there’s no better cleanup and decontamination company than Bio-One of Charlotte. 

    We carry out every job with courtesy and trust—by helping first—then tending to business afterwards. As a close-knit crew, we devote our time and energy to cleaning and decontaminating what others won’t or can’t do. No job is too big or challenging for us to see through to the end.

    We’re experts in Charlotte emergency services cleanup, hoarding cleanup, and more, with every technician on our crew receiving proper certification to clean crime scenes as well as appropriate training to follow all OSHA guidelines.

    So, put your trust in a cleanup company that goes above and beyond for you when you need it most, without fail.

    Call Us 27/4
    Your Local Cleanup Partner

    Rooted in the community. Driven by compassion.

    The person behind your local Bio-One isn’t just a business owner—they’re a neighbor who cares deeply about helping others through life’s most challenging moments. Trained, certified, and ready to respond 24/7, they lead a team committed to doing the right thing with discretion, empathy, and professionalism.

    You can trust that when you call, you get real help from someone who truly understands.

    John and Jen Symons

    John and Jen Symons are the owners of Bio-One of Charlotte. Both John and Jen grew up in Michigan and moved to Charlotte, NC in 2007. John has worked in the restoration field for over ten years, and loves helping his customers. He uses his experience and expertise to help restore their client's homes to a safe and clean condition. Jen graduated from Central Michigan University and is passionate about helping those in need. John and Jen have two daughters and are very active in their community of Rock Hill, SC.
    Learn more about me and my mission
    Call Us 24/7

    Real People. Real Compassion.

    Our local team is trained, experienced, and here to help—no judgment, just support.

    We understand that calling us means something serious has happened. That’s why our local Bio-One team brings more than training—we bring compassion, clear communication, and respect every step of the way.

    • Highly trained and experienced technicians
    • Honest, in-person estimates
    • Discreet and professional service
    • Locally owned and operated

    You can count on us to handle it all—with care.

    Meet my team

    What our clients say

    decontamination and biohazard cleaning services reviews
    devon lowe, Charlotte, 03/2024
    John, Jen, and their team are fantastic! They helped my family after the passing of my father. They went way beyond what I thought I hired them for and even let me cry on their shoulder. If you are in need of these services, you absolutely should hire them!
    decontamination and biohazard cleaning services reviews
    Emily Shirley, Charlotte, 03/2024
    Amazing group of people who are so incredibly professional and compassionate. Logan, Nikki, and Ash kept me updated throughout the entire process and were all so unbelievably helpful, I never felt embarrassed or shamed (Even my dogs loved them!) They all did such an amazing job and I am SO grateful for the service they completed in a very quick and efficient manner. Ruth’s communication throughout everything was stellar and she is such an amazing person. The entire process from the walkthrough to the cleaning was quick and simple. Thank y’all so much!
    decontamination and biohazard cleaning services reviews
    Kaci Shea, Charlotte, 03/2024
    Where do I even BEGIN. These wonderfully amazing people helped me gain control of my Life back. 10 years of hoarding and they cleared my home out in 3 days. Every single one of them we’re compassionate and kind. They talked me through the process each step of the way. Ruth helped me gain my confidence back to push through all of this. Matt was so kind and thoughtful. Joey and I talked about dnd and video games and it felt nice to be able to Smile and laugh through a tough process. Blaze and Ashe were so kind and helped any time I had a question. I only got to meet John and Nikki once but they were so sweet and thoughtful towards my anxiety about everything. It was truly an honor to have all them by my side through this hardship. I hope they see this and realize how much this truly meant to me. Thank you… you gave me and my mom control back over our lives. You all are truly amazing people. And I hope they universe blesses you with everything and anything 🖤.
    COMMUNITY

    Rooted in the Community, Ready to Help

    We live here, we care, and we’re proud to serve our neighbors.

    We love the people, places, and spirit of the communities we serve. That’s why we show up 24/7—to help our neighbors through tough moments with care, compassion, and respect.

    From local families to first responders, we’re honored to be a trusted part of the community.
    Serving Charlotte and Surrounding Areas
    Find your nearest Bio-One office

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    Frequently asked questions

    What types of situations does Bio-One handle?
    We clean biohazards, trauma scenes, suicides, unattended deaths, hoarding situations, and more—discreetly and with care.
    Are you really available 24/7?
    Yes. No matter the time or day, someone from our local team is always ready to answer your call.
    Will my information be kept private?
    Absolutely. Upon request, we arrive in unmarked vehicles and treat every situation with total discretion and respect.
    How quickly can you arrive?
    We respond quickly—usually the same day. We’re locally owned, so we can get to you quickly.
    Do you work with insurance?
    Yes. We’ll work directly with your insurance provider to help make the process easier for you.
    What happens when I call Bio-One?
    A kind, trained expert will listen, explain the next steps, and guide you through the process—no pressure, no judgment.
    Is your team trained for hazardous cleanup?
    Yes. Our technicians are fully trained, certified, and experienced in handling dangerous and sensitive situations.
    Do I have to be home during the cleanup?
    Not if you don’t want to be. We’ll work with your schedule and comfort level every step of the way.
    How long does cleanup usually take?
    It depends on the situation, but most cleanups take a day or two. We’ll give you a clear timeline upfront.
    Why should I trust Bio-One?
    Because we genuinely care. We’re part of your community and show up with compassion, professionalism, and respect—every time.