Compassion You Can Feel. Professionalism You Can Trust.

When life takes an unexpected turn, you shouldn’t have to face it alone. Bio-One is here to help—quietly, respectfully, and with genuine care. Every Bio-One location is locally owned by people who care deeply about their community.

Why People Choose Bio-One: 

  • Help First, Business Second. Compassion leads every call.
  • 24/7 Immediate Response. Real people, any time.
  • Certified Experts. OSHA and IICRC trained.
  • Discreet and Respectful. Privacy always protected.
  • Locally Owned, Nationally Backed.

You’re never alone—Bio-One is ready to help, day or night. Call 414- 418-2521

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How Bio-One Stands Above the Rest

When it comes to sensitive cleanup, “clean” isn’t enough—it must be done completely, safely, and with care. Bio-One has earned the trust of families and first responders nationwide by leading with compassion and precision.

What Makes Bio-One Different:

Comprehensive Cleanup

We don’t stop at surfaces—we remediate what you can’t see.

Insurance Coordination

We handle the paperwork and guide you through every step.

Trusted by Law Enforcement

Professionals rely on our discretion and proven expertise.

Why Choose Bio-One

Specialized Expertise
We set the industry standard in crime and trauma scene cleanup, biohazard remediation, hoarding cleanup, and other related services.

Mission-Driven Approach
We operate with the motto: "Help First, Business Second," prioritizing clients' emotional needs during traumatic events.

Highly Experienced
Trusted leader in biohazard remediation with over 20 years of experience.

Trusted by Authorities
Clients include police, fire, victim advocates, funeral homes, and hospitals.

Comprehensive Services
Offers a wide range of services including crime scene, suicide, undiscovered death, hoarding, medical waste disposal, and mold remediation.

Discretion and Compassion
Provides complete discretion, often using unmarked vehicles, and handles all situations with care and compassion.

Professional and Trained Staff
Experienced and trained teams with clear, supportive communication and a comprehensive cleaning approach.

Insurance Collaboration
Assists clients by collaborating with insurance companies.

Locally Owned
Each office is independently owned and operated, providing community-centric service.

Competitive Pricing/Value
Known for outstanding service at a competitive price and providing good value, especially for remediation work like odor removal.

Real Stories, Real Impact: What Our Clients Say

The impact of our work is best told by those we've helped. Our clients—families, businesses, and first responders—share how Bio-One provided professional, compassionate service during life’s most challenging moments.

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Bio-One is a true lifeline in difficult times.

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Daniel Levin Madison, WI

Trusted By

9870 Satisfied Customers

What our clients say

decontamination and biohazard cleaning services reviews
Carol C., Waukesha, 03/2025
Bio-One did a great job for me. Very professional and they knew what to do. Came in on time and budget! I had a horrible mouse infestation that had been going on for years. Lots of mess and damage and they cleaned it all up. Plus, they consulted with me, ensuring that my mouse problem was eradicated before they came in to do the cleanup so that my effort would not be wasted. Very reliable highly recommend!
decontamination and biohazard cleaning services reviews
Sean M., Waukesha, 11/2024
Andrew and team were great We had a wonderful experience with them. Had a rodent infestation that was taken care of but had significant amount of leftover feces and urine in basement. They cleaned everything from top to bottom. Got rid of ruined furniture for us Looks amazing and clean Exceptional work and would recommend to anyone who needs this service
decontamination and biohazard cleaning services reviews
Jim M., Waukesha, 10/2024
Getting rid of things you've amassed over a lifetime can not only be difficult and deeply personal, but knowing who to trust to handle it for you can add to the stress. Andrew and the crew at Bio-One of Milwaukee made everything, and I do mean EVERYTHING a breeze. From the initial estimate to the last box out the door, the Bio-One folks were all thorough and professional. Perhaps most importantly, they were deeply concerned on a personal level that the experience was stress-free and everything I needed it to be, including the fact that the owner was on-site with his crew the whole time! Bio-One is not a low-cost provider, but you will not regret the minimal extra expense to have your cleanup and removal handled with their amazing quality of service. If had known that there was someone of Andrew and his crew's caliber available, I would have hired them years earlier. Not joking. Years! Call Andrew. Get an estimate. It will only take 5 minutes of chatting with him to realize that you made the right call. Thanks Bio-One!

The Bio-One Promise

Every Bio-One service begins with empathy. Before tools or training, we focus on people—helping them through moments they never imagined. “Help First, Business Second” isn’t a slogan; it’s how we show up—day or night—with patience, care, and respect.

Our Commitment to You

  • We respond fast, ready to help anytime.
  • We treat every person and space with dignity.
  • We meet the highest safety and professional standards.
  • We communicate clearly and compassionately.

We stay until your peace of mind is restored.
That’s our promise—Help first. Business second. Always.

We respond 24/7.
Request a service now.

    Here When You Need Us Most

    Three clear steps. One caring, professional team.
    1

    Reach Out Anytime—24/7

    Call whenever you're ready. A compassionate, trained specialist will listen, answer your questions, and walk you through what happens next.
    2

    We Arrive Ready to Help

    Our team responds quickly, assesses the situation alongside you, and builds a thoughtful plan—always with discretion and respect for your space.
    3

    Compassionate Cleanup, Start to Finish

    We take care of everything with care and precision, keeping you informed along the way. We’ll also coordinate with your insurance provider to help reduce your stress.

    Get Expert Help When You Need It Most

    Call Us 24/7

    When safety, confidentiality, and experience matter most, trust Bio-One. Contact us for a free consultation and let our certified professionals help restore peace of mind.

    Recognized Excellence, Certified Expertise

    Bio-One sets the standard in biohazard cleanup—not only through compassion and care but also through specialized training and certification. 

    Our technicians are professionally trained, licensed, and certified to safely handle even the most complex biohazard situations. That commitment has earned us recognition from leading industry organizations, trusted media outlets, and the communities we serve daily.

    What Sets Us Apart:

    • Certified Technicians trained in OSHA, bloodborne pathogens, and industry best practices
    • Licensed Professionals who follow strict safety and sanitation protocols
    • Proven Track Record of success with thousands of clients served
    • Trusted Nationwide with features in media and endorsements from local communities
    • Compassionate, Respectful Service from the first call to final cleanup

    Frequently asked questions

    What types of situations does Bio-One handle?
    We clean biohazards, trauma scenes, suicides, unattended deaths, hoarding situations, and more—discreetly and with care.
    Are you really available 24/7?
    Yes. No matter the time or day, someone from our local team is always ready to answer your call.
    Will my information be kept private?
    Absolutely. Upon request, we arrive in unmarked vehicles and treat every situation with total discretion and respect.
    How quickly can you arrive?
    We respond quickly—usually the same day. We’re locally owned, so we can get to you quickly.
    Do you work with insurance?
    Yes. We’ll work directly with your insurance provider to help make the process easier for you.
    What happens when I call Bio-One?
    A kind, trained expert will listen, explain the next steps, and guide you through the process—no pressure, no judgment.
    Is your team trained for hazardous cleanup?
    Yes. Our technicians are fully trained, certified, and experienced in handling dangerous and sensitive situations.
    Do I have to be home during the cleanup?
    Not if you don’t want to be. We’ll work with your schedule and comfort level every step of the way.
    How long does cleanup usually take?
    It depends on the situation, but most cleanups take a day or two. We’ll give you a clear timeline upfront.
    Why should I trust Bio-One?
    Because we genuinely care. We’re part of your community and show up with compassion, professionalism, and respect—every time.
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