Bio-One decontamination and biohazard cleaning services


Blood Spill Clean Up

Blood is known to be a vessel for many infectious diseases. The improper cleaning of such spills could lead to Hepatitis, HIV, and other bloodborne pathogens. That’s where we step in. Let Bio-One clean it for you.

You called the right 
team for the job.

Blood spills can pose a major safety threat to anyone near the impacted area. Bio-One team members are experts on cleaning and all areas impacted by biohazards. We will answer your call 24/7 and can respond quickly to be there for you.

Trained Technicians

Our cleanup teams are expertly trained and adhere to OSHA guidelines. We have experience disinfecting residential, commercial, and industrial locations affected by crime scenes.

Bio-One - Bio Hazard DecontaminationBio-One Team always ready to help.
Bio-One Expert Services

Expert Services

With trained technicians and state-of-the-art tools, we can handle any biohazard job. Residential, commercial, or industrial, we have the training and experience you are looking for.
Bio-One Discreet & Safe

Discreet & Safe

Discretion and confidentiality are important for all our clients. Our teams will arrive and complete the job in a thorough and safe manner.
Bio-One Compassion & Competence

Compassion & Competence

Help first, business second is our motto. Our team leads with compassion and serves our clients with respect and professionalism.
Bio-One Available 24/7

Available 24/7

Bio-One is here for you whenever you need us. You will receive 24/7 access to a local company with nationwide expertise.
Bio-One Insurance Assistance

Insurance Assistance

Working with your insurance company can be overwhelming. Bio-One can open the claim and work with the adjusters directly.
Bio-One Trusted Response Team

Trusted Response Team

A quick response can make all the difference when it comes to biohazards. Our teams will respond in an urgent manner, ready to help.

Available 24/7

We are here for you when you need us. For any Blood Spill Clean Up situation, we are available 24/7.  Whatever you need and whenever you need us, know that we will be supportive, professional and discrete.

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decontamination and biohazard cleaning services reviews
User Reviews
Bio-One changed my life. I was looking for a way to serve my community and provide for my family at the same time, when I came across this franchise opportunity. I bought my first territory after finding the corporate team to be like-minded and values driven. The community of franchisees has been an invaluable source of encouragement, support and wisdom on this journey. Three years later, I bought my second franchise that is doing even better than the first!
Travis Nichols, Littleton, 02/09/2021
decontamination and biohazard cleaning services reviews
User Reviews
At the worst time of my families life, this company made what was anticipated to be the worst thing in the world to do the easiest part of this tragedy. From contacting the company and speaking with Mark to his team everything was handled professionally, with empathy, and respectfully. Chase and Chloe's response at the house was liken to having your friends come help you deal with a terrible situation. They made me feel supported, they were informative and made what I feared to be the worst part of dealing with a suicide the easiest so far. I highly recommend this company that helps you deal with the cleanup as well as other things you just don't know about when someone dies in this manner. I rarely do reviews because it's not often I feel so passionate about something a business has done. This company exceeded my expectations and truly does what no one else ever wants to do. I'm beyond grateful for the as individuals and a company!
Catherine Wilson, Littleton, 04/20/2021
decontamination and biohazard cleaning services reviews
User Reviews
Outstanding service!!! Very professional and thorough. The issue was resolved promptly and communication could not have been better. I highly recommend.
Gordon Smith, Littleton, 03/27/2021
decontamination and biohazard cleaning services reviews
User Reviews
Reached out to Bio-One asking for help on a BAD odor and explained that it was an emergency as it was in a church. The gentleman that answered the phone not only answered my questions but also assured me that they would be able to work on it that day and do whatever they could so Sunday Morning services could take place. Spoke with them the next morning and they relayed to me that the church was able to continue with services as planned and that everything was freshened up. HIGHLY RECOMMEND them for any all your Bio Needs.
Sara Thompson, Littleton, 10/14/2021
decontamination and biohazard cleaning services reviews
User Reviews
They did a great job cleaning up a mess that our renter made in his bedroom. They were very professional.They were also very friendly. Great job !!!
Kelli Elliott, Littleton, 02/01/2019
decontamination and biohazard cleaning services reviews
User Reviews
I recently hired Bio-One to clean out a relative's 2000 square foot house which they occupied for over 60 years. A lot of "stuff" had accumulated over the years, not only in the house but the shed too. Throw in a case of hoarding by the remaining daughter and her son, along with her sick cat that urinated on the carpeting. Due to the daughter's hoarding, infestation of cockroaches were found throughout the kitchen. Once the house was vacated Bio-One moved in to discard everything not nailed down in the house along with all carpeting. After emptying the house they deep cleaned every square inch including the insides of all cabinets, appliances and bathrooms too. The before and after pictures are a sight to be seen. He (Cuyler) and his team did it four plus days. Yes, we attempted to clean it out ourselves (five adults) but after three weekends and not making a dent, we had no choice. Bio-One sent us daily "progress pictures" along with keeping us in the loop with multiple text messages. His proposal was extremely detailed with what they were to do so there weren't any questions. Totally amazed and would highly recommend.
Gregg Prudence, Littleton, 02/06/2020
decontamination and biohazard cleaning services reviews
User Reviews
After many years in the real estate business we decided to look for a business that would feel more rewarding. We wanted to give back to our community. We researched numerous franchise opportunities and kept coming back to Bio-One, Inc. Boy are we glad we did! We have been in business a little over a year. The support is outstanding. Proud to say we far surpassed our expectations for 2020...and 2021 is looking even better!
Chris Phillips, Littleton, 02/03/2021
decontamination and biohazard cleaning services reviews
User Reviews
Fast, friendly, professional, knowledgeable service at a more than reasonable price.
Gail Frazier Gordon, Littleton, 03/12/2019
decontamination and biohazard cleaning services reviews
User Reviews
Marc was very helpful and knows his stuff. He was able to identify areas of pet damage and eleminate it. He was fast and efficient. If I need help in the future, Bio-One is who I'll call.
Theodore Reichert, Littleton, 11/02/2021
decontamination and biohazard cleaning services reviews
User Reviews
Fast, friendly, professional, knowledgeable service at a more than reasonable price.
Gail Frazier Gordon, Littleton, 03/12/2019

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Bio-One decontamination and biohazard cleaning services